Regulation of Lysosomal Associated Membrane Protein 3 (LAMP3) in Lung Epithelial Cells by Coronaviruses (SARS-CoV-1/2) and Type I Interferon Signaling

Abstract Severe Acute Respiratory Syndrome CoronaVirus-2 (SARS-CoV-2) infection is a major risk factor for mortality and morbidity in critical care hospitals around the world. Lung epithelial type II cells play role recognition clearance of respiratory viruses as well repair lung injury response to environmental toxicants. Gene expression profiling studies revealed that mouse express several cell-specific markers including surfactant proteins Lysosomal associated membrane protein 3 (LAMP3) located lysosomes, endosomes lamellar bodies. These intracellular organelles are involved vesicular transport facilitate viral entry release genome into host cell cytoplasm. In this study, regulation LAMP3 human by I interferon signaling was investigated. SARS-CoV-1 SARS-CoV-2 significantly induced within 24 hours after required presence ACE2 receptors. Time course experiments correlated with Interferon–beta (IFNB1) STAT1 at mRNA levels. also direct IFN-beta treatment multiple lines or influenza virus lacking non-structural protein1(NS1) NHBE bronchial cells. RSV HPIV3. Location lysosomes aswell induction Interferon suggests may have an important inter-organellar innate immunity potential target therapeutic modulation health disease. Furthermore, bioinformatics subset genes were differentially regulated lungs COVID-19 patients.